List of overrepresented sequences from Bisulfite Sequencing run with Larvae files. This entry lists the possible source, sequence, and percentage of possible overrepresented sequences/adapters.

From November Run:

F R1: TruSeq Adapter, Index 3 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC (1.02%) No Hit AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG (0.67%) TruSeq Adapter, Index 3 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC (0.47%) No Hit GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA (0.20%) F R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (1.03%) No Hit TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC (0.31%) Illumina Single End PCR Primer 1 (100% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT (0.28%) Illumina Single End PCR Primer 1 (98% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGT (0.12%) No Hit ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC (0.10%) T3D3 R1: TruSeq Adapter, Index 6 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC (1.97%) TruSeq Adapter, Index 6 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC (0.65%) T3D3 R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (2.02%) Illumina Single End PCR Primer 1 (100% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT (0.40%) Illumina Single End PCR Primer 1 (98% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGT (0.15%) T1D3 R1: TruSeq Adapter, Index 4 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC (1.48%) TruSeq Adapter, Index 4 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC (0.23%) T1D3 R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (1.51%) Illumina Single End PCR Primer 1 (100% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT (0.15%) T1D5 R1: TruSeq Adapter, Index 5 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC (0.60%) TruSeq Adapter, Index 5 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC (0.25%) T1D5 R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (0.62%) Illumina Single End PCR Primer 1 (100% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT (0.17%) T3D5 R1: TruSeq Adapter, Index 7 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC (1.23%) TruSeq Adapter, Index 7 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC (0.19%) T3D5 R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (1.28%) Illumina Single End PCR Primer 1 (100% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT (0.19%) M1 R1: TruSeq Adapter, Index 8 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC (1.39%) TruSeq Adapter, Index 8 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC (0.44%) M1 R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (1.41%) Illumina Single End PCR Primer 1 (100% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT (0.26%) Illumina Single End PCR Primer 1 (98% over 50bp) ATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGT (0.11%) M3 R1: TruSeq Adapter, Index 9 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC (0.75%) M3 R2: Illumina Single End PCR Primer 1 (100% over 50bp) GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG (0.65%)

From September Run:

F R1: No Hit AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG (1.00%) No Hit GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA (0.29%) TruSeq Adapter, Index 3 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC (0.21%) TruSeq Adapter, Index 3 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC (0.18%) No Hit TGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG (0.13%) F R2: No Hit TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC (0.36%) No Hit ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC (0.11%) T3D3 R1: TruSeq Adapter, Index 6 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC (0.73%) TruSeq Adapter, Index 6 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC (0.35%) T3D3 R2: No overrepresented sequences T1D3 R1: TruSeq Adapter, Index 4 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC (0.35%) T1D3 R2: No overrepresented sequences T1D5 R1: TruSeq Adapter, Index 5 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC (0.20%) TruSeq Adapter, Index 5 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC (0.13%) T1D5 R2: No overrepresented sequences T3D5 R1: TruSeq Adapter, Index 7 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC (0.52%) TruSeq Adapter, Index 7 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC (0.12%) T3D5 R2: No overrepresented sequences M1 R1: TruSeq Adapter, Index 8 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC (0.38%) TruSeq Adapter, Index 8 (100% over 50bp) ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC (0.21%) M1 R2: No overrepresented sequences M3 R1: TruSeq Adapter, Index 9 (100% over 50bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC (0.33%) M3 R2: No overrepresented sequences
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